The National Science Foundation (NSF) and Japan Science and Technology Agency (JST) have issued new joint awards to reduce pesticide pollution and promote biofuel.

In particular, James Liao of the UCLA Henry Samueli School of Engineering and Applied Science and Eiichiro Fukusaki of Osaka University have been awarded $1.5 million over three years for their joint research project.

The biofuel known as butanol–which can be produced by fermentation processes involving bacteria and yeast–provides particular promise because it can be used to fuel cars. Liao and Fukusaki will genetically engineer photosynthetic bacteria to maximize butanol production based on comprehensive metabolic analyses of bacteria and yeast and on computational modeling.

Liao and Fukusaki’s team is one of four joint United States and Japanese research teams that were awarded funding totaling about $12 million to develop new environmentally-friendly techniques to increase the production of renewable biofuel and reduce pesticide use.

These environmentally-friendly techniques will be based on studies of metabolites–chemical compounds that are produced in all living cells and are integral to a wide range of important and life-sustaining biological processes, including the ability of plants to fight disease-causing pests and the ability of photosynthetic algae to produce biofuel.

Currently, scientists can identify and characterize the properties of only a small fraction of the 10,000 to 15,000 metabolites that exist in any given plant. Nevertheless, an improved ability to identify and characterize these pivotal compounds could lead to the development of entirely new and potentially breakthrough approaches for increasing biofuel production and reducing pesticide use.

The four research projects are not only important for their potential to help increase biofuel production and reduce pesticide use but also for their potential to produce much needed advances in the overall field of metabolomics–the study of all of an organism’s metabolites. Metabolomics has exciting applications in varied fields, including environmental science, synthetic biology, medicine and predictive modeling of plant, algal and microbial systems.
NSF and JST issued the four awards under the new “Metabolomics for a Low Carbon Society” program, the first to be fully jointly coordinated by the two agencies.

Each project will last three years, but could be extended for another two years based on the results of an evaluation that will be conducted during the third year of the project.

The four new Metabolomics for a Low Carbon Society projects will capitalize on the technical expertise and research infrastructures of both the United States and Japan, and to promote collaborative research between scientists in the two countries.

To this end, NSF and JST required all proposals for funding to include at least one U.S. researcher and one Japanese researcher, and NSF and JST jointly reviewed all submitted proposals.

“Projects from this program will benefit from complementary expertise, innovative approaches and cooperation–the kinds of advantages provided by international collaborations,” said Subra Suresh, the director of NSF. “These projects offer particular promise for producing cutting-edge results that will take the field of metabolomics to new levels and yield important environmental benefits.”

“The goal of this joint NSF-JST program is to advance novel biological knowledge in metabolomics in the areas of energy and the environment,” said Michiharu Nakamura, the president of JST. “It is expected that the collaborative research among U.S. and Japanese scientists focused on fundamental research will strengthen a broader understanding of metabolomics, which is one of the most advanced areas of life science. It is also expected that the outcome of the joint research will significantly contribute to the development of a low carbon society.”

Although each of the four funded trans-Pacific research projects addresses a unique research problem, these projects collectively address widespread challenges underlying metabolomics in all of its applications. These challenges include the pressing need to identify more metabolites and to develop more flexible and sophisticated metabolomics infrastructures, including comprehensive databases of species-dependent metabolites.  By addressing these widespread problems, results of the four funded projects will likely broadly advance the entire field.

The three other new research projects are as follows:

  • Research led by Lloyd W. Sumner of The Samuel Roberts Nobel Foundation and Kazuki Saito of the RIKEN Plant Science Center. This project will leverage advanced instrumentation to identify and characterize important metabolites related to biomass and oil production in plants. Also, the broad metabolic effects of gene-level changes affecting plant cell walls and oil synthesis will be identified. Through these advances, the project will inform efforts to improve plant cell wall material fermentability, produce energy-rich plant oils and provide resources for other metabolomics researchers.
  • Research led by Oliver Fiehn of the University of California at Davis and Masanori Arita of the University of Tokyo. This project will improve methods to identify metabolites used by photosynthetic algae to produce biofuel–information that will be used to boost the manufacturing of biofuel. Project activities include advancing techniques in mass spectrometry–a method used to estimate the mass of molecules–and developing a new, integrated mass spectrometry database that will be freely accessible to the research community.
  • Research led by Georg Jander of the Boyce Thompson Institute for Plant Research in New York and Yutaka Okumoto of Kyoto University. Up to 30 percent of agricultural productivity is lost to insects and disease. Moreover, production costs soar every time a tractor drives over a field to apply pesticides. This project will help reduce such losses and costs by supporting the development of technologies that will better define natural disease-fighting processes in plants. As part of this effort, protective metabolites that stop attacks by insects and other disease-causing organisms in plants and genes responsible for producing these metabolites will be identified.

Joint funding for Metabolomics for a Low Carbon Society is operated by NSF under its Division of Integrative Organismal Systems, Division of Molecular and Cellular Biosciences and Division of Biological Infrastructure, and is operated by JST under its Strategic International Collaborative Research Program (SICORP).

JST is Japan’s core organization for implementing science and technology policy in line with the objectives of Japan’s Science and Technology Basic Plan. The organization is dedicated to establishing Japan as a nation built on the creativity of science, technology and innovation. SICORP supports international exchange/joint research projects in various fields with counterpart agencies of other countries via international agreements.

Main Image: A type of photosynthetic bacteria that will be studied as part of the Liao/Fukusaki project, which will be devoted to increasing butanol production. Photo by Hidevaldo Machado, UCLA.